A novel two-step procedure to expand cardiac Sca-1+ cells clonally
نویسندگان
چکیده
منابع مشابه
Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration.
Recent studies have shown that cardiac stem cells (CSCs) from the adult mammalian heart can give rise to functional cardiomyocytes; however, the definite surface markers to identify a definitive single entity of CSCs and the molecular mechanisms regulating their growth are so far unknown. Here, we demonstrate a single-cell deposition analysis to isolate individually selected CSCs from adult mur...
متن کاملAdult cardiac Sca-1-positive cells differentiate into beating cardiomyocytes.
Although somatic stem cells have been reported to exist in various adult organs, there have been few reports concerning stem cells in the heart. We here demonstrate that Sca-1-positive (Sca-1+) cells in adult hearts have some of the features of stem cells. Sca-1+ cells were isolated from adult murine hearts by a magnetic cell sorting system and cultured on gelatin-coated dishes. A fraction of S...
متن کاملIdentification and Characterization of a Novel Multipotent Sub-Population of Sca-1+ Cardiac Progenitor Cells for Myocardial Regeneration
METHODS AND RESULTS The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and low Sca-1 (CSL1; 37.1%; CSL2; 17.4%) expressing cells were selected for further studies. Sca-1⁺ cells...
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In recent decades, one of the challenges in sheet metal forming was production of two-step bending surfaces without mechanical tools and external force or by a combination of heat source and mechanical tools. Forming with a heat source such as laser beam has the potential for forming arbitrary 3D shapes such as two-step bending surfaces. In this paper a novel method for laser forming of complic...
متن کاملDifferentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells
Cardiac resident stem/progenitor cells (CSC/CPCs) are critical to the cellular and functional integrity of the heart because they maintain myocardial cell homeostasis. Several populations of CSC/CPCs have been identified based on expression of different stem cell-associated antigens. Sca-1(+) cells in the cardiac tissue may be the most common CSC/CPCs. However, they are a heterogeneous cell pop...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2007
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2007.05.216